TRANSLATIONAL HISTOPATHOLOGY LABORATORY (THL)

Immunofluorescence Projects

Immunohistochemistry Projects

The Translational Histopathology Laboratory (THL) is based in the Old Road Campus Research Building next to the Churchill Hospital. It specialises in the translation of research into clinically accredited and deployable Immunohistochemistry (IHC) and Immunofluorescence (IF) assays. The lab is supported by funds from the NIHR/BRC and CRUK. It is led by Drs Sarah Blagden and Alistair Easton.

The goal of the THL is to establish GCP compliant and validated assays for biomarkers that can be used to accompany the development of new cancer treatments. We work with research teams in the preclinical development of IHC biomarkers in mouse or human tissue which can then be further developed to accompany a clinical trial (to GCP standard). Alternatively, we can conduct IHC on samples (such as archived or fresh tumour tissue) obtained from clinical trial patients.

The facility is available as a core service to any Oxford University or OUHFT based cancer researcher for the following:

  • Tissue processing, embedding and sectioning
  • Haematoxylin & Eosin (H&E) Staining
  • Antibody optimisation
  • Single and double antibody tissue staining
  • Slide scanning and scoring
  • TMA construction
  • RNA Scope
  • Multi-plex Immunofluorescence (IF) imaging. Use of the PerkinElmer Polaris system allows up to 6 epitopes to be assayed in a single tissue section. These can be tailored to your project or selected from off the shelf kits. The same system can be used to determine the localisation of several RNA molecules in a single sample.

If you are interested in using any of the above services please email ecmc@oncology.ox.ac.uk. Alternatively, the application form for accessing THL services can be accessed from our website and emailed to the same address.